Searching for key regulatory woodiness genes in the giant cabbage

Brassica cross

In collaboration with Institute of Biology at Leiden University, we have set up a tissue culture line of a cross starting from two grandparent lines: the giant woody Jersey kale and the small herbaceous TO1000, both belonging to the same species Brassica oleracea. The cross of two F1 genotypes has generated 192 F2 accessions. We are focusing on phenotyping woodiness and drought tolerance in the F2 population. These traits will be genetically mapped to find Quantitative Trait Loci (QTLs), or genomic regions that are associated with the traits of interest. Together with future genomic and transcriptomic analyses along with CRISPR-based mutagenesis, we aim to find a number of key regulatory genes involved in wood formation in cabbage and other derived woody lineages in Brassicaceae.